382
382 .
MINERAL MEDIUM M9 for E. coli JM strains
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
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Medium for Escherichia coli DSM 6721
This strain uses the standard medium without modifications.
Main sol. 382 #
ml
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| 10 x M9 salts (per l) | 100 | ml | - | - | |
|
MgSO4
(1 M) |
1 | ml | 0.12 | 1 | |
|
CaCl2
(0.1 M) |
1 | ml | 0.011 | 0.1 | |
|
Thiamine-HCl x 2 H2O
(filter-sterilized, 1 M) |
1 | ml | 0.373 | 1 | |
|
Glucose
(20%) |
10 | ml | 2 | 11.101 | |
| Distilled water | 900 | ml | - | - | |
| 1 The above solutions should be sterilized separately by filtration (thiamine, glucose) or autoclaving. | |||||
| 2 Adjust pH to 7.4. | |||||
Metadata
| Last modified: | 09.03.23 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 7.4 |
| Equipment needed: | Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 13 |
| Strains with modifications: | 1 |
Escherichia coli DSM 6721
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na2HPO4 | 6 | 42.266 |
| KH2PO4 | 3 | 22.045 |
| Glucose | 2 | 11.101 |
| NH4Cl | 1 | 18.695 |
| NaCl | 0.5 | 8.556 |
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Cultivation metadata from 13 strains
| phyla | 1 | 13 strains |
| temperature | mesophilic | 3 strains |