354 .
LÖWENSTEIN-JENSEN MEDIUM
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Selected strain: Mycobacterium senegalense DSM 43664
This strain uses the standard medium without modifications.
Growth conditions: 37 °C
Equipment needed:
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 2.50 | g | ||
|
|
MgSO4 | 0.24 | g | ||
|
|
Mg-citrate | 0.60 | g | ||
|
|
L-Asparagin | 3.60 | g | ||
|
|
Potato flour | 30.00 | g | ||
|
|
Malachite green | 0.40 | g | ||
|
|
Distilled water | 600.00 | ml | ||
| 1 Addition: | |||||
|
|
Glycerol | 12.00 | ml | ||
|
|
Fresh egg mixture
(yolk and whites, *) |
1000.00 | ml | ||
| 2 pH 7.0 | |||||
| 3 Sterilize medium for 30 min at 121°C, cool to 30°C and add fresh egg mixture carefully to the medium. | |||||
| 4 * You need for 1 l of egg mixture 20 - 25 fresh eggs (not older then 1 week). Clean the eggs, wipe away with 70% alcohol, open the eggs and homogenize the egg mixture carefully. | |||||