302 .
NUTRIENT BROTH WITH 10% HORSE SERUM
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Selected strain: Alysiella crassa DSM 2578
This strain uses the standard medium without modifications.
Growth conditions: 37 °C
Equipment needed:
Autoclave
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Peptone | 5 | g | ||
|
|
Meat extract | 3 | g | ||
|
|
Agar , if necessary | 15 | g | ||
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Distilled water | 1000 | ml | ||
| 1 To autoclaved medium add: | |||||
|
|
Horse serum
(sterile) |
100 | ml | ||
| 2 Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation. | |||||