This medium was extracted automatically and has not been curated by experts, yet.
This strain uses the standard medium without modifications.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Wilkins-Chalgren-Anaerobe-Broth
(Oxoid) |
1000.0 | ml | - | - | |
L-Cysteine | 0.3 | g | 0.3 | 2.476 | |
Resazurin | 1.0 | mg | 1.0e-3 | 0.004 | |
Sterile serum
(horse, bovine or lamb) |
50.0 | ml | - | - | |
1 Wilkins-Chalgren-Anaerobe-Broth (Oxoid) is supplemented with 0.3 g/l of L-cysteine and 1.0 mg/l of resazurin. Prepare the medium under 100% nitrogen gas. To the autoclaved medium add 50 ml/l of sterile serum (horse, bovine or lamb). |
Last modified: | 18.05.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | n.d. |
Equipment needed: | Autoclave, Gassing station, Hungate tubes |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 2 |
No modifications necessary for this strain.
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
L-Cysteine | 0.3 | 2.476 |
Resazurin | 1.0e-3 | 0.004 |
Wilkins-Chalgren-Anaerobe-Broth | - | - |
Sterile serum | - | - |