248 .
OAT FLAKES AGAR WITH YEAST EXTRACT
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Selected strain: Actinobifida chromogena DSM 43367
This strain uses the standard medium without modifications.
Growth conditions: 50 °C
Equipment needed:
Filter
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Oat flakes | 20 | g | ||
|
|
Trace element solution | 1 | ml | ||
|
|
Yeast extract | 2 | g | ||
|
|
Agar | 20 | g | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Adjust pH to 7.2. | |||||
| 2 Boil oat flakes for 20 min, filter through cheese cloth and adjust to 1000 ml. Add trace elements and agar. | |||||
Trace element solution #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
FeSO4 x 7 H2O | 0.1 | g |
|
|
MnCl2 x 4 H2O | 0.1 | g |
|
|
ZnSO4 x 7 H2O | 0.1 | g |
|
|
Distilled water | 100.0 | ml |