246 .
SEA WATER AGAR
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Selected strain: Actinopolyspora sp. DSM 41877
This strain uses the standard medium without modifications.
Growth conditions: 37 °C
Equipment needed:
Autoclave
Final pH:
7.3
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Beef extract | 10 | g | ||
|
|
Peptone | 10 | g | ||
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Agar | 20 | g | ||
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Tap water | 250 | ml | ||
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Sea water | 750 | ml | ||
| 1 Dissolve beef extract and peptone by heating in tap water, adjust pH to 7.8 and boil for 10 min. Readjust pH to 7.3. Add agar and autoclave at 121°C for 20 min. Directly after autoclaving, add warm (55°C) sterile sea water. Liquid medium without agar should be combined when cooled to room temperature. | |||||
| 2 Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water. | |||||
Artificial sea water #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaCl | 28.13 | g |
|
|
KCl | 0.77 | g |
|
|
CaCl2 x 2 H2O | 1.60 | g |
|
|
MgCl2 x 6 H2O | 4.80 | g |
|
|
NaHCO3 | 0.11 | g |
|
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MgSO4 x 7 H2O | 3.50 | g |
|
|
Distilled water | 1000.00 | ml |