223 .
POLYANGIUM MEDIUM
What is this page?
Selected strain: Sorangium cellulosum DSM 2276
This strain uses the standard medium without modifications.
Growth conditions: 26 °C
Equipment needed:
Autoclave
Filter
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KNO3 | 1.00 | g | ||
|
|
K2HPO4 | 1.00 | g | ||
|
|
MgSO4 x 7 H2O | 0.30 | g | ||
|
|
CaCl2 x 2 H2O | 0.13 | g | ||
|
|
FeCl3 x 6 H2O | 32.00 | mg | ||
|
|
Agar | 10.00 | g | ||
|
|
Tap water | 1000.00 | ml | ||
| 1 Adjust pH to 7.2 (use pH indicator-paper). | |||||
| 2 Aseptically add one or more sterile strips of filter paper to slants or plates. Inoculate the filter papers with a small (5 mm x 5mm) agar cube or a piece of filter paper overgrown with the strain. | |||||