219 .
MYCOBACTERIUM MEDIUM
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Selected strain: Hoyosella subflava DSM 45089
This strain uses the standard medium without modifications.
Growth conditions: 28 °C
Equipment needed:
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract | 2.0 | g | ||
|
|
Proteose peptone no. 3 | 2.0 | g | ||
|
|
Casein peptone
(tryptic digested) |
2.0 | g | ||
|
|
Na2HPO4 x 12 H2O | 2.5 | g | ||
|
|
KH2PO4 | 1.0 | g | ||
|
|
Sodium citrate | 1.5 | g | ||
|
|
MgSO4 x 7 H2O | 0.6 | g | ||
|
|
Glycerol | 50.0 | ml | ||
|
|
Tween | 0.5 | g | ||
|
|
Agar | 20.0 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Adjust pH to 7.0. | |||||