215c .
BHI MEDIUM FOR STRICT ANAEROBES
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Selected strain: Roseburia porci DSM 107448
This strain uses the standard medium without modifications.
Growth conditions: 2-3 days
aerobic
37 °C
pH 7.00
Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
7.2 - 7.6
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ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Brain heart infusion
(DIFCO) |
37.00 | g | ||
|
|
L-Cysteine HCl x H2O | 0.25 | g | ||
|
|
Na2S x 9 H2O | 0.25 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Prepare medium anoxically under 100% N2 gas atmosphere. Reducing agents are added after autoclaving from sterile anoxic solutions prepared under N2. | |||||