1a .
REACTIVATION WITH LIQUID MEDIUM 1
What is this page?
Selected strain: Xanthomonas perforans DSM 18975
This strain uses the standard medium without modifications.
Growth conditions: 28 °C
Equipment needed:
Final pH:
7.0
Select a strain (optional):
ml
| 1 Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 1. Subsequent subculturing may be carried out in liquid medium or on agar plates. | |||||
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Peptone | 5 | g | ||
|
|
Meat extract | 3 | g | ||
|
|
Agar , for solid medium | 15 | g | ||
|
|
Distilled water | 1000 | ml | ||
| 2 Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation. | |||||