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DSMZ

DSMZ 194 . DESULFOBULBUS SP. MEDIUM (FRESHWATER)

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Medium for Desulfofundulus sp. DSM 7475

* Modification: Na-propionate is replaced by 0.40 g/l 3,4,5-trimethoxybenzoic acid added after autoclaving from a sterile anoxic stock solution (2% w/v) prepared under N2 and neutralized with NaOH. Stock solutions of 3,4,5-trimethoxybenzoate should be prepared freshly and sterilized by filtration. Supplement medium with 1.00 ml/l seven vitamins solution (see medium 503) added from an anoxic stock solution sterilized by filtration.

Growth conditions: 3-7 days anaerobic 55 °C

Main sol. 194 #

ml
Equipment needed: Autoclave Gassing station Filter Hungate tubes
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 952 ml - -
Solution B 30 ml - -
Solution C* 20 ml - -
Solution D 1 ml - -
Solution E 10 ml - -
Seven vitamins solution* 1 ml - -
1 Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclaved. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solutions C and E are autoclaved under 100% N2 gas. Solution D is prepared under 100% N2 gas atmosphere and sterilized by filtration. Solutions B to E are added to the sterile, cooled solution A in appropriate amounts in the sequence as indicated. Final pH of the medium should be 7.1 - 7.4.
2 Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% (v/v) inoculum.

Solution A #

952
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2SO4 3.00 g 3.151 22.186
KH2PO4 0.20 g 0.21 1.544
NH4Cl 0.30 g 0.315 5.891
NaCl 1.00 g 1.05 17.974
MgCl2 x 6 H2O 0.40 g 0.42 2.067
KCl 0.50 g 0.525 7.045
CaCl2 x 2 H2O 0.15 g 0.158 1.072
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
Sodium resazurin
(0.1% w/v) 		0.50 ml 5.3e-4 0.002
Distilled water 950.00 ml - -

Solution B #

30
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 1.5 g 50 471.752
Distilled water 30.0 ml - -

Solution C #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-propionate* 1.5 g 150 1561.52
3,4,5-Trimethoxybenzoic acid* 0.4 g - -
Distilled water* 20.0 ml - -

Solution D #

1
Compound Amount Unit Conc. [g/L] Conc. [mM]
Wolin's vitamin solution (10x) 1 ml - -

Solution E #

10
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2S x 9 H2O 0.4 g 40 166.54
Distilled water 10.0 ml - -

Trace element solution SL-10 #

1000
Solution occurs in 266 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Selenite-tungstate solution #

1000
Solution occurs in 179 other media (first defined in Medium 385).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
Distilled water 1000.0 ml - -

Wolin's vitamin solution (10x) #

1000
Solution occurs in 306 other media (first defined in Medium 120).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Pyridoxine hydrochloride 100 mg 0.1 0.486
Thiamine HCl 50 mg 0.05 0.148
Riboflavin 50 mg 0.05 0.133
Nicotinic acid 50 mg 0.05 0.406
Calcium D-(+)-pantothenate 50 mg 0.05 0.21
Vitamin B12 1 mg 1.0e-3 7.4e-4
p-Aminobenzoic acid 50 mg 0.05 0.365
(DL)-alpha-Lipoic acid 50 mg 0.05 0.242
Distilled water 1000 ml - -

Seven vitamins solution * #

1000
Solution occurs in 78 other media (first defined in Medium 503).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
D-(+)-biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 1.459
Thiamine-HCl x 2 H2O 200 mg 0.2 0.536
Distilled water 1000 ml - -

Metadata

Last modified: 06.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.1 - 7.4
Equipment needed: Autoclave, Filter, Gassing station, Hungate tubes
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Gas composition

  • 80 % N2
  • 20 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 34
Strains with modifications: 25
Desulfofundulus sp. DSM 7475

  Na-propionate
  Distilled water
  0.40 g 3,4,5-Trimethoxybenzoic acid
  20 ml Distilled water
+  1.0 ml Seven vitamins solution
+  20 ml Solution C
-  Solution C

Molecular composition
* modified beta

Compound Conc. [g/L] Conc. [mM]
Na2SO4 2.991 21.058
Na2CO3 1.496 14.11
NaCl 0.997 17.06
KCl 0.499 6.687
MgCl2 x 6 H2O 0.399 1.962
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Cultivation metadata from 34 strains BacDive

pH 6 - 8 3 strains
phyla 3 34 strains
oxygen anaerobe 29 strains
oxygen obligate anaerobe 1 strain
temperature mesophilic 30 strains
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