193
193 .
DESULFOBACTER MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Paracoccus solventivorans DSM 6637
* Modification: Replace Na-acetate with 0.58 g/l acetone. Supplement medium with 3.40 g/l KNO3 and omit solution E. Add after autoclaving 5.00 g/l Na-pyruvate from an anoxic stock solution sterilized by filtration.
Growth conditions: facultative anaerobic
30 °C
Main sol. 193 #
ml
Equipment needed: Autoclave
Gassing station
Hungate tubes
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Solution A | 942 | ml | - | - | |
| Solution B | 30 | ml | - | - | |
| Solution C | 20 | ml | - | - | |
| Solution D | 1 | ml | - | - | |
| Solution E* | 10 | ml | - | - | |
| 1 Solution A is sparged with 80% N2 and 20% CO2 gas mixture to reach a pH below 6 (at least 30 min), then distributed under the same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclaved. Solution B is autoclaved separately under 80% N2 and 20% CO2 gas atmosphere. Solutions C and E are autoclaved under 100% N2 gas. Solution D is prepared under 100% N2 gas atmosphere and sterilized by filtration. To complete the medium appropriate amounts of solutions B to E are added to the sterile solution A in the sequence as indicated. Final pH of the medium should be 7.1 - 7.4. | |||||
| 2 Note: Addition of 10 - 20 mg sodium dithionite per liter (e.g. from 5% (w/v) solution, freshly prepared under N2 and filter-sterilized) may stimulate growth of some strains at the beginning. For transfers use 5 - 10% (v/v) inoculum. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Na2SO4 | 3.00 | g | 3.185 | 22.421 |
| KH2PO4 | 0.20 | g | 0.212 | 1.56 |
| NH4Cl | 0.30 | g | 0.318 | 5.954 |
| NaCl | 7.00 | g | 7.431 | 127.156 |
| MgCl2 x 6 H2O | 1.30 | g | 1.38 | 6.788 |
| KCl | 0.50 | g | 0.531 | 7.12 |
| CaCl2 x 2 H2O | 0.15 | g | 0.159 | 1.083 |
| Trace element solution SL-10 | 1.00 | ml | - | - |
| Selenite-tungstate solution | 1.00 | ml | - | - |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.3e-4 | 0.002 |
| KNO3* | 3.40 | g | - | - |
| Na-pyruvate* | 5.00 | g | - | - |
| Distilled water | 940.00 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Na2CO3 | 1.5 | g | 50 | 471.752 |
| Distilled water | 30.0 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Na-acetate x 3 H2O* | 2.50 | g | 125 | - |
| Acetone* | 0.58 | g | - | - |
| Distilled water | 20.00 | ml | - | - |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Na2S x 9 H2O | 0.4 | g | 40 | 166.54 |
| Distilled water | 10.0 | ml | - | - |
Solution occurs in 179
other media (first defined in Medium 385).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| NaOH | 0.5 | g | 0.5 | 12.501 |
| Na2SeO3 x 5 H2O | 3.0 | mg | 0.003 | 0.011 |
| Na2WO4 x 2 H2O | 4.0 | mg | 0.004 | 0.012 |
| Distilled water | 1000.0 | ml | - | - |
Solution occurs in 266
other media (first defined in Medium 320).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HCl
(25%) |
10.0 | ml | 2.5 | 68.568 | |
| FeCl2 x 4 H2O | 1.5 | g | 1.5 | 7.545 | |
| ZnCl2 | 70.0 | mg | 0.07 | 0.514 | |
| MnCl2 x 4 H2O | 100.0 | mg | 0.1 | 0.505 | |
| H3BO3 | 6.0 | mg | 0.006 | 0.097 | |
| CoCl2 x 6 H2O | 190.0 | mg | 0.19 | 1.463 | |
| CuCl2 x 2 H2O | 2.0 | mg | 0.002 | 0.012 | |
| NiCl2 x 6 H2O | 24.0 | mg | 0.024 | 0.185 | |
| Na2MoO4 x 2 H2O | 36.0 | mg | 0.036 | 0.149 | |
| Distilled water | 990.0 | ml | - | - | |
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Solution occurs in 306
other media (first defined in Medium 120).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Biotin | 20 | mg | 0.02 | 0.082 |
| Folic acid | 20 | mg | 0.02 | 0.045 |
| Pyridoxine hydrochloride | 100 | mg | 0.1 | 0.486 |
| Thiamine HCl | 50 | mg | 0.05 | 0.148 |
| Riboflavin | 50 | mg | 0.05 | 0.133 |
| Nicotinic acid | 50 | mg | 0.05 | 0.406 |
| Calcium D-(+)-pantothenate | 50 | mg | 0.05 | 0.21 |
| Vitamin B12 | 1 | mg | 1.0e-3 | 7.4e-4 |
| p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 |
| (DL)-alpha-Lipoic acid | 50 | mg | 0.05 | 0.242 |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 06.09.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 7.1 - 7.4 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 80 % N2
- 20 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Molecular composition
* modified
beta
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 6.979 | 119.423 |
| Na-pyruvate | 4.985 | 45.301 |
| KNO3 | 3.39 | 33.529 |
| Na2SO4 | 2.991 | 21.058 |
| Na2CO3 | 1.496 | 14.11 |
| Show more | ||
Cultivation metadata from 15 strains
| pH | 6 - 8 | 1 strain |
| pH | 8 - 10 | 1 strain |
| phyla | 3 | 15 strains |
| oxygen | anaerobe | 14 strains |
| oxygen | facultative anaerobe | 1 strain |
| Show more | ||