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SELENOMONAS RUMINANTIUM MEDIUM
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Selected strain: Selenomonas ruminantium subsp. lactilytica DSM 2872
This strain uses the standard medium without modifications.
Growth conditions: 37 °C
Equipment needed:
Autoclave
Hungate tubes
Final gas composition:
100 % CO2
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Final pH:
7.0
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ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Glucose | 1.0 | g | ||
|
|
Trypticase
(BBL) |
5.0 | g | ||
|
|
KH2PO4 | 1.0 | g | ||
|
|
Na-acetate | 4.0 | g | ||
|
|
Yeast extract | 2.0 | g | ||
|
|
n-Valeric acid | 0.1 | ml | ||
|
|
Resazurin | 1.0 | mg | ||
|
|
Na2CO3 | 4.0 | g | ||
|
|
L-Cysteine HCl x H2O | 0.5 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Adjust pH to 7.0. Gas atmosphere: 100% CO2. | |||||
| 2 Distribute into Hungate tubes and autoclave. | |||||