172 .
CYTOPHAGA (marine) MEDIUM
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Selected strain: Marivirga tractuosa DSM 4126
This strain uses the standard medium without modifications.
Growth conditions: 25 °C
Equipment needed:
Autoclave
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract
(Difco) |
1.0 | g | ||
|
|
Tryptone
(Difco) |
1.0 | g | ||
|
|
NaCl | 24.7 | g | ||
|
|
KCl | 0.7 | g | ||
|
|
MgSO4 x 7 H2O | 6.3 | g | ||
|
|
MgCl2 x 6 H2O | 4.6 | g | ||
|
|
CaCl2 x 2 H2O | 1.2 | g | ||
|
|
NaHCO3 | 0.2 | g | ||
|
|
Agar
(Difco) |
15.0 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Adjust pH to 7.2. Sodium bicarbonate and calcium chloride are autoclaved separately, each in a small volume of distilled water. | |||||