1678
1678 .
Cyanobacteria Medium BA+50+B+N/2
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Medium for Leptolyngbya sp. DSM 104174
* Modification: Standing culture, 16 hours at 50 lux illumination, 8-hours dark period. Transfer interval of 8 weeks
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 23 °C
Main sol. 1678 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
NaCl
(250 g/l stock solution) |
33.300 | g/l | 33.3 | 569.815 | |
|
MgCl2 x 6 H2O
(200 g/l stock solution) |
3.300 | g/l | 3.3 | 16.232 | |
|
KCl
(50 g/l stock solution) |
3.300 | g/l | 3.3 | 44.266 | |
|
Mg(SO4) x 7 H2O
(350 g/l stock solution) |
3.300 | g/l | 3.3 | - | |
|
CaCl2 x 2 H2O
(50 g/l stock solution) |
3.300 | g/l | 3.3 | 22.447 | |
|
Na3-citrate x 2 H2O
(0.6 g/l stock solution) |
8.300 | ml/l | 0.005 | - | |
|
Na2-EDTA x 2 H2O
(0.1 g/l stock solution) |
8.300 | ml/l | 8.3e-4 | 0.002 | |
| T-Trace Metal Mix | 1.000 | ml | - | - | |
| Distilled water | 1000.000 | ml | - | - | |
| 1 Adjust to 1000 mL with H2O dist. and autoclave. | |||||
| 2 After cooling, add the following filter sterilized components: | |||||
|
NaNO3
(300 g/l stock solution) |
2.075 | ml/l | 0.623 | 7.324 | |
|
K2HPO4 x 3 H2O
(8.0 g/l stock solution) |
4.150 | ml/l | 0.033 | 0.191 | |
|
NaHCO3
(52.5 g/l stock solution) |
3.430 | ml/l | 0.18 | 2.144 | |
|
Fe(NH4)citrate
(6.0 g/l stock solution) |
0.800 | ml/l | 0.005 | - | |
|
NH4Cl
(53.5 g/l stock solution) |
0.500 | ml/l | 0.027 | 0.5 | |
|
Vitamin B12
(0.02 g/l stock solution) |
1.000 | ml/l | 2.0e-5 | 1.5e-5 | |
Solution occurs in 1
other media (first defined in Medium 1672).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| ZnSO4 x 7 H2O | 0.22 | g/l | 0.22 | 0.765 |
| MnCl2 x 4 H2O | 1.81 | g/l | 1.81 | 9.146 |
| H3BO3 | 2.86 | g/l | 2.86 | 46.248 |
| Na2MoO4 x 2 H2O | 0.39 | g/l | 0.39 | 1.612 |
|
CuSO4 x 5 H2O
(0.8 g/100mL stock sol.) |
10.00 | ml/l | - | - |
|
Co(NO3)2 x 6 H2O
(0.5 g/L stock sol.) |
10.00 | ml/l | 0.005 | 0.017 |
Metadata
| Last modified: | 24.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | n.d. |
| Edit in Medium builder | |
Light
| Intensity | 50 lux lux |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 33.3 | 569.815 |
| KCl | 3.3 | 44.266 |
| CaCl2 x 2 H2O | 3.3 | 22.447 |
| MgCl2 x 6 H2O | 3.3 | 16.232 |
| Mg(SO4) x 7 H2O | 3.3 | - |
| Show more | ||
Cultivation metadata from 17 strains
| phyla | 1 | 17 strains |
| temperature | psychrophilic | 17 strains |