This medium was extracted automatically and has not been curated by experts, yet.
* Modification: Standing culture, 16 hours at 60 lux illumination, 8-hours dark period. Transfer interval of 8 weeks
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl
(250 g/l stock solution) |
33.300 | g/l | 33.3 | 569.815 | |
MgCl2 x 6 H2O
(200 g/l stock solution) |
3.300 | g/l | 3.3 | 16.232 | |
KCl
(50 g/l stock solution) |
3.300 | g/l | 3.3 | 44.266 | |
Mg(SO4) x 7 H2O
(350 g/l stock solution) |
3.300 | g/l | 3.3 | - | |
CaCl2 x 2 H2O
(50 g/l stock solution) |
3.300 | g/l | 3.3 | 22.447 | |
Na3-citrate x 2 H2O
(0.6 g/l stock solution) |
8.300 | ml/l | 0.005 | - | |
Na2-EDTA x 2 H2O
(0.1 g/l stock solution) |
8.300 | ml/l | 8.3e-4 | 0.002 | |
T-Trace Metal Mix | 1.000 | ml | - | - | |
Distilled water | 1000.000 | ml | - | - | |
1 Adjust to 1000 mL with H2O dist. and autoclave. | |||||
2 After cooling, add the following filter sterilized components: | |||||
NaNO3
(300 g/l stock solution) |
2.075 | ml/l | 0.623 | 7.324 | |
K2HPO4 x 3 H2O
(8.0 g/l stock solution) |
4.150 | ml/l | 0.033 | 0.191 | |
NaHCO3
(52.5 g/l stock solution) |
3.430 | ml/l | 0.18 | 2.144 | |
Fe(NH4)citrate
(6.0 g/l stock solution) |
0.800 | ml/l | 0.005 | - | |
NH4Cl
(53.5 g/l stock solution) |
0.500 | ml/l | 0.027 | 0.5 | |
Vitamin B12
(0.02 g/l stock solution) |
1.000 | ml/l | 2.0e-5 | 1.5e-5 |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
ZnSO4 x 7 H2O | 0.22 | g/l | 0.22 | 0.765 |
MnCl2 x 4 H2O | 1.81 | g/l | 1.81 | 9.146 |
H3BO3 | 2.86 | g/l | 2.86 | 46.248 |
Na2MoO4 x 2 H2O | 0.39 | g/l | 0.39 | 1.612 |
CuSO4 x 5 H2O
(0.8 g/100mL stock sol.) |
10.00 | ml/l | - | - |
Co(NO3)2 x 6 H2O
(0.5 g/L stock sol.) |
10.00 | ml/l | 0.005 | 0.017 |
Last modified: | 24.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | n.d. |
Edit in Medium builder |
Intensity | 60 lux lux |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 33.3 | 569.815 |
KCl | 3.3 | 44.266 |
CaCl2 x 2 H2O | 3.3 | 22.447 |
MgCl2 x 6 H2O | 3.3 | 16.232 |
Mg(SO4) x 7 H2O | 3.3 | - |
Show more |
phyla | 1 | 17 strains |
temperature | psychrophilic | 17 strains |