1677
1677 .
Cyanobacteria Medium BA+
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Cyanobacteria sp. DSM 104200
* Modification: Standing culture, 16 hours at 140 lux illumination, 8-hours dark period. Transfer interval of 8 weeks
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 23 °C
Main sol. 1677 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
NaCl
(250 g/l stock solution) |
33.30 | g | 33.3 | 569.815 | |
|
MgCl2 x 6 H2O
(200 g/l stock solution) |
3.30 | g | 3.3 | 16.232 | |
|
KCl
(50 g/l stock solution) |
3.30 | g | 3.3 | 44.266 | |
|
Mg(SO4) x 7 H2O
(350 g/l stock solution) |
3.30 | g | 3.3 | - | |
|
CaCl2 x 2 H2O
(50 g/l stock solution) |
3.30 | g | 3.3 | 22.447 | |
|
Na3-citrate x 2 H2O
(0.6 g/l stock solution) |
8.30 | ml | - | - | |
|
Na2-EDTA x 2 H2O
(0.1 g/l stock solution) |
8.30 | ml | - | - | |
| T-Trace Metal Mix * | 1.00 | ml | - | - | |
| 1 Make up to 1000 ml with MilliQ water. | |||||
| 2 Autoclave at 121 °C for 20 min. | |||||
| 3 After cooling, add the following filter sterilized components: | |||||
|
NaNO3
(300 g/l stock solution) |
4.15 | ml | - | - | |
|
K2HPO4 x 3 H2O
(8.0 g/l stock solution) |
4.15 | ml | - | - | |
|
Na2CO3
(20 g/l stock solution) |
2.30 | ml | 5.819 | 54.902 | |
|
Fe(NH4)citrate
(6.0 g/l stock solution) |
0.80 | ml | - | - | |
|
Vitamin B12
(0.02 g/l stock solution) |
1.00 | ml | - | - | |
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| ZnSO4 x 7 H2O | 0.22 | g/l | 0.22 | 0.765 | |
| MnCl2 x 4 H2O | 1.81 | g/l | 1.81 | 9.146 | |
| H3BO3 | 2.86 | g/l | 2.86 | 46.248 | |
| Na2MoO4 x 2 H2O | 0.39 | g/l | 0.39 | 1.612 | |
|
CuSO4 x 5 H2O
(0.08 g/l stock solution) |
10.00 | ml/l | 8.0e-4 | 0.003 | |
|
Co(NO3)2 x 6 H2O
(0.05 g/l stock solution) |
10.00 | ml/l | 5.0e-4 | 0.002 | |
| 1 Make up to 1000 ml with MilliQ water. | |||||
Metadata
| Last modified: | 30.01.24 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Light
| Intensity | 140 lux lux |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 33.3 | 569.815 |
| Na2CO3 | 5.819 | 54.902 |
| KCl | 3.3 | 44.266 |
| CaCl2 x 2 H2O | 3.3 | 22.447 |
| MgCl2 x 6 H2O | 3.3 | 16.232 |
| Show more | ||
Cultivation metadata from 18 strains
| phyla | 1 | 15 strains |
| temperature | psychrophilic | 14 strains |