1673
1673 .
Cyanobacteria Medium ASN 3+
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Phormidium formosum DSM 101194
* Modification: Standing culture, 16 hours at 160 lux illumination, 8-hours dark period. Transfer interval of 8 weeks
The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 18 °C
Main sol. 1673 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
NaCl
(250 g/l stock solution) |
100.0 | ml/l | 25 | 427.789 | |
|
MgCl2 x 6 H2O
(200 g/l stock solution) |
10.0 | ml/l | 2 | 9.838 | |
|
KCl
(50 g/l stock solution) |
10.0 | ml/l | 0.5 | 6.707 | |
|
Mg(SO4) x 7 H2O
(350 g/l stock solution) |
10.0 | ml/l | 3.5 | - | |
|
CaCl2 x 2 H2O
(50 g/l stock solution) |
10.0 | ml/l | 0.5 | 3.401 | |
|
Na3-citrate x 2 H2O
(0.6 g/l stock solution) |
5.0 | ml/l | 0.003 | - | |
|
Na2-EDTA x 2 H2O
(0.1 g/l stock solution) |
5.0 | ml/l | 5.0e-4 | 0.001 | |
| T-Trace Metal Mix | 1.0 | ml | - | - | |
| 1 Make up to 1000 ml with MilliQ water. | |||||
| 2 After cooling, add the following filter-sterilized components: | |||||
|
NaNO3
(300 g/l stock solution) |
2.5 | ml/l | 0.75 | 8.824 | |
|
K2HPO4 x 3 H2O
(8.0 g/l stock solution) |
2.5 | ml/l | 0.02 | 0.115 | |
|
Na2CO3
(20 g/l stock solution) |
1.0 | ml/l | 0.02 | 0.189 | |
|
Fe(NH4)citrate
(6.0 g/l stock solution) |
0.5 | ml/l | 0.003 | - | |
|
Vitamin B12
(0.02 g/l stock solution) |
1.0 | ml/l | 2.0e-5 | 1.5e-5 | |
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| H3BO3 | 2.90 | g/l | 2.9 | 46.895 |
| MnCl2 x 4 H2O | 1.81 | g/l | 1.81 | 9.146 |
| ZnSO4 x 7 H2O | 0.22 | g/l | 0.22 | 0.765 |
| Na2MoO4 x 2 H2O | 0.39 | g/l | 0.39 | 1.612 |
|
CuSO4 x 5 H2O
(0.08 g/l stock solution) |
10.00 | ml/l | 8.0e-4 | 0.003 |
|
Co(NO3)2 x 6 H2O
(0.05 g/l stock solution) |
10.00 | ml/l | 5.0e-4 | 0.002 |
Metadata
| Last modified: | 19.12.23 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | n.d. |
| Edit in Medium builder | |
Light
| Intensity | 160 lux lux |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 25 | 427.789 |
| Mg(SO4) x 7 H2O | 3.5 | - |
| MgCl2 x 6 H2O | 2 | 9.838 |
| NaNO3 | 0.75 | 8.824 |
| KCl | 0.5 | 6.707 |
| Show more | ||
Cultivation metadata from 17 strains
| phyla | 2 | 16 strains |
| temperature | psychrophilic | 13 strains |