1571 .
MICROBACTER MEDIUM
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Selected strain: Microbacter margulisiae DSM 27471
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
30 °C
Equipment needed:
Autoclave
Final pH:
5.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
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KH2PO4 | 0.41 | g | ||
|
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Na2HPO4 x 2 H2O | 0.53 | g | ||
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NH4Cl | 0.30 | g | ||
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NaCl | 0.30 | g | ||
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MgCl2 x 6 H2O | 0.10 | g | ||
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CaCl2 x 2 H2O | 0.01 | g | ||
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Yeast extract | 2.00 | g | ||
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Na2S x 9 H2O | 0.36 | g | ||
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Resazurin | 0.50 | g | ||
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Trace element solution SL-10 | 1.00 | ml | ||
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Distilled water | 1000.00 | ml | ||
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L-Cysteine | 0.50 | g | ||
| 1 Dissolve in 1 liter, boil under N2, add 0.5 g of L-cysteine and adjust pH to 6.4 (should be pH 5.5 after autoclaving). Disperse in tubes and bottles (leave 50% of container volume as gas phase). Change to gas mixture N2/CO2; 80/20%) and autoclave for 20 minutes at 121°C. | |||||
| 2 To 100 ml sterile medium add aseptically | |||||
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Vitamin solution | 0.50 | ml | ||
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Selenite-tungstate solution | 0.50 | ml | ||
Trace element solution SL-10 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
HCl
(25%) |
10.0 | ml | ||
|
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FeCl2 x 4 H2O | 1.5 | g | ||
|
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ZnCl2 | 70.0 | mg | ||
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MnCl2 x 4 H2O | 100.0 | mg | ||
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H3BO3 | 6.0 | mg | ||
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CoCl2 x 6 H2O | 190.0 | mg | ||
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CuCl2 x 2 H2O | 2.0 | mg | ||
|
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NiCl2 x 6 H2O | 24.0 | mg | ||
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Na2MoO4 x 2 H2O | 36.0 | mg | ||
|
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Distilled water | 990.0 | ml | ||
| 1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml. | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
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Folic acid | 2.0 | mg |
|
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Pyridoxine hydrochloride | 10.0 | mg |
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Thiamine-HCl x 2 H2O | 5.0 | mg |
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Riboflavin | 5.0 | mg |
|
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Nicotinic acid | 5.0 | mg |
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D-Calcium pantothenate | 5.0 | mg |
|
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Vitamin B12 | 0.1 | mg |
|
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p-Aminobenzoic acid | 5.0 | mg |
|
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Lipoic acid | 5.0 | mg |
|
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Distilled water | 1000.0 | ml |
Selenite-tungstate solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaOH | 0.5 | g |
|
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Na2SeO3 x 5 H2O | 3.0 | mg |
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Na2WO4 x 2 H2O | 4.0 | mg |
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Distilled water | 1000.0 | ml |