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DSMZ

DSMZ 1567 . METHYLOPROFUNDUS SEDIMENTI MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
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Medium for Methyloprofundus sedimenti DSM 29215

This strain uses the standard medium without modifications.

Growth conditions: 20 °C

Main sol. 1567 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution 1 (10x NMS salts) 100.0 ml - -
Solution 2 Phosphate buffer (10x) 10.0 ml - -
Solution 3 Trace element solution 1.0 ml - -
Solution 4 Vitamin solution 1.0 ml - -
Solution 5 Sodium chloride (15x) 66.0 ml - -
Solution 6 (Fe EDTA) 0.4 ml - -
Distilled water 1000.0 ml - -
1 Bring to 1 liter with distilled water.
2 Dilute 100 ml solution 1 to 1 litre with distilled water and then add 1 ml of solution 3, 66 ml solution 5 and 0.4 ml of solution 6. Dispense the medium into the growth vessels. If using sealed vessels it is appropriate to add 50% methane to the gas phase and autoclave at 121C (15psi) for 15 minutes. Autoclave the phosphate buffer separately. Filter sterilized the vitamin solution. When the growth medium is cool 1.0 ml/l vitamin solution and 10 ml/l of the phosphate buffer are added - if too warm the phosphate will precipitate. Liquid cultures should be grown with shaking.

Solution 1 (10x NMS salts) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
KNO3 10 g 10 98.909
MgSO4 x 6 H2O 10 g 10 83.078
CaCl2 x 2 H2O 2 g 2 13.604
Distilled water 1000 ml - -
1 Dissolve the ingredients listed above (in that order) in about 700 ml of distilled water, and then make up to 1 litre.

Solution 2 Phosphate buffer (10x) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 12 H2O 71.6 g 71.6 504.371
KH2PO4 26.0 g 26 191.057
Distilled water 1000.0 ml - -
1 Dissolve in about 800 ml of water, adjust the pH to 6.8 and make up to 1 litre.

Solution 3 Trace element solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Final pH 7.0 (with KOH).

Solution 4 Vitamin solution #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Biotin 2.0 mg 0.002 0.008
Folic acid 2.0 mg 0.002 0.005
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Thiamine-HCl x 2 H2O 5.0 mg 0.005 0.013
Riboflavin 5.0 mg 0.005 0.013
Nicotinic acid 5.0 mg 0.005 0.041
D-Calcium pantothenate 5.0 mg 0.005 -
Vitamin B12 0.1 mg 1.0e-4 7.4e-5
p-Aminobenzoic acid 5.0 mg 0.005 0.036
Lipoic acid 5.0 mg 0.005 0.024
Distilled water 1000.0 ml - -

Solution 5 Sodium chloride (15x) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 300 g 300 5133.47
Distilled water 1000 ml - -

Solution 6 (Fe EDTA) #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Fe(III)-EDTA 3.8 g 3.8 11.045
Distilled water 1000.0 ml - -

Metadata

Last modified: 15.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: n.d.
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Gas composition

  • 50 % CH4

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1
Methyloprofundus sedimenti DSM 29215

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 19.8 338.809
KNO3 1 9.891
MgSO4 x 6 H2O 1 8.308
Na2HPO4 x 12 H2O 0.716 5.044
KH2PO4 0.26 1.911
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature psychrophilic 1 strain