153 .
THIOGLYCOLATE MEDIUM (MERCK 108190)
What is this page?
Selected strain: Propionispora hippei DSM 15287
This strain uses the standard medium without modifications.
Growth conditions: anaerobic
37 °C
Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final gas composition:
100 % N2
read more
Final pH:
7.1
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Trypticase
(BBL) |
15.0 | g | ||
|
|
L-Cystine | 0.5 | g | ||
|
|
Glucose | 2.0 | g | ||
|
|
Yeast extract | 5.0 | g | ||
|
|
NaCl | 2.5 | g | ||
|
|
Na-thioglycolate | 0.5 | g | ||
|
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | ||
|
|
Agar
, for solid medium
optional |
15.0 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Dissolve ingredients, sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Adjust pH to 7.1 with Na2CO3 (5% w/v), if necessary. Then dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. | |||||