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DSMZ

DSMZ 1536 . BAP+ MEDIUM FOR FRANKIA

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
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Medium for Frankia casuarinae DSM 45818

This strain uses the standard medium without modifications.

Growth conditions: more than 4 weeks aerobic 28 °C pH 6.30

Main sol. 1536 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.950 g 0.95 6.981
K2HPO4 0.600 g 0.6 3.445
NH4Cl 0.270 g 0.27 5.048
Na-propionate 0.480 g 0.48 4.997
MgSO4 x 7 H2O 0.025 g 0.025 0.101
CaCl2 x 2 H2O 0.010 g 0.01 0.068
Vitamine solution 1.000 ml - -
Chelated iron solution 1.000 ml - -
Trace element solution 0.100 ml - -
Distilled water 1000.000 ml - -
1 The pH of the final solution is adjusted to 6.3.
2 Note: Please autoclave the chelated iron solution before you add to the medium, so that solves everything!!!!

Vitamine solution #

100
Solution occurs in 3 other media (first defined in Medium 1536).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Thiamine HCl 0.01 g 0.1 0.296
Nicotinic acid 0.05 g 0.5 4.061
Pyridoxine hydrochloride 0.05 g 0.5 2.431
Distilled water 100.00 ml - -

Chelated iron solution #

100
Solution occurs in 3 other media (first defined in Medium 1536).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Citric acid 1 g 10 52.051
Eisencitrat 1 g 10 40.328
Distilled water 100 ml - -

Trace element solution #

100
Solution occurs in 3 other media (first defined in Medium 1536).
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
CoSO4 x 7 H2O 1.000 mg 0.01 0.065
CuSO4 x 5 H2O 0.080 g 0.8 3.204
H3BO3 2.860 g 28.6 462.484
MnCl2 x 4 H2O 1.810 g 18.1 91.458
Na2MoO4 x 2 H2O 0.025 g 0.25 1.033
ZnSO4 x 7 H2O 0.220 g 2.2 7.651
Distilled water 100.000 ml - -

Metadata

Last modified: 19.09.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.3
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 14
Frankia casuarinae DSM 45818

No modifications necessary for this strain.

Molecular composition

Compound Conc. [g/L] Conc. [mM]
KH2PO4 0.95 6.981
K2HPO4 0.6 3.445
Na-propionate 0.48 4.997
NH4Cl 0.27 5.048
MgSO4 x 7 H2O 0.025 0.101
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Cultivation metadata from 14 strains BacDive

pH 6 - 8 5 strains
phyla 2 13 strains
nutrition chemoorganotroph 4 strains
nutrition heterotroph 4 strains
oxygen aerobe 4 strains
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