150a .
ACIDITHIOBACILLUS CALDUS MEDIUM
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Selected strain: Acidithiobacillus caldus DSM 8584
This strain uses the standard medium without modifications.
Growth conditions: 2-3 days
45 °C
Equipment needed:
Autoclave
Filter
Water bath
Final pH:
2.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
(NH4)2SO4 | 3.00 | g | ||
|
|
K2HPO4 x 3 H2O | 0.50 | g | ||
|
|
MgSO4 x 7 H2O | 0.50 | g | ||
|
|
KCl | 0.10 | g | ||
|
|
Ca(NO3)2 x 4 H2O | 0.02 | g | ||
|
|
Trace element solution | 10.00 | ml | ||
|
|
Sulfur
(powdered) |
5.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve ingredients except trace elements and sulfur. Adjust pH with 6 N H2SO4 to 2.5, dispense into suitable cultivation vessels and autoclave. After autoclaving add filter-sterilized trace elements solution (see below) and sterile sulfur powder. For sterilization place the sulfur in screw-capped tubes or bottles, wet with a few drops of water and heat for 3 hours to 90 - 100°C in a water bath on each of three successive days. Before use, aseptically layer the sulfur onto the surface of the autoclaved liquid basal medium. | |||||
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
FeCl3 x 6 H2O | 1.10 | g |
|
|
CuSO4 x 5 H2O | 0.05 | g |
|
|
H3BO3 | 0.20 | g |
|
|
MnSO4 x H2O | 0.20 | g |
|
|
Na2MoO4 x 2 H2O | 0.08 | g |
|
|
CoCl2 x 6 H2O | 0.06 | g |
|
|
ZnSO4 x 7 H2O | 0.09 | g |
|
|
Distilled water | 1000.00 | ml |