1403
1403 .
METHYLOROSULA MEDIUM V-022
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
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Medium for Methylorosula polaris DSM 22001
This strain uses the standard medium without modifications.
Growth conditions: 20 °C
Main sol. 1403 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 0.05 | g | 0.05 | 0.378 | |
| MgCl2 x 6 H2O | 0.04 | g | 0.04 | 0.197 | |
| KH2PO4 | 0.07 | g | 0.07 | 0.514 | |
| Trace Element Solution | 1.00 | ml | - | - | |
| Distilled water | 1000.00 | ml | - | - | |
| Methanol | 0.50 | %(v/v) | - | - | |
| 1 Prepare the liquid medium. Adjust to pH 6.0. Dispense the medium in tubes or flasks fitted with screw caps. Sterilize by autoclaving. Before inoculation add filter sterilized CH3OH to a final concentration of 0.5% v/v. | |||||
Equipment needed: Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Na2-EDTA | 500 | mg | 0.5 | 1.487 |
| FeSO4 x 7 H2O | 200 | mg | 0.2 | 0.719 |
| ZnSO4 x 7 H2O | 10 | mg | 0.01 | 0.035 |
| MgCl2 x 6 H2O | 3 | mg | 0.003 | 0.015 |
| H3BO3 | 30 | mg | 0.03 | 0.485 |
| CoCl2 x 6 H2O | 20 | mg | 0.02 | 0.154 |
| CaCl2 x 2 H2O | 1 | mg | 1.0e-3 | 0.007 |
| NiCl2 x 6 H2O | 2 | mg | 0.002 | 0.015 |
| Na2MoO4 x 2 H2O | 3 | mg | 0.003 | 0.012 |
| Distilled water | 1000 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Defined medium |
| Final pH: | 6.0 |
| Equipment needed: | Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Methylorosula polaris DSM 22001
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| KH2PO4 | 0.07 | 0.514 |
| (NH4)2SO4 | 0.05 | 0.378 |
| Na2-EDTA | 5.0e-4 | 0.001 |
| FeSO4 x 7 H2O | 2.0e-4 | 7.2e-4 |
| H3BO3 | 3.0e-5 | 4.9e-4 |
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