13 .
BACILLUS ACIDOCALDARIUS MEDIUM
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* Medium for Alicyclobacillus kakegawensis DSM 17979
Plus MnSO4 (see medium 1); pH:4.2
Growth conditions: 50 °C
Equipment needed:
Autoclave
Final pH:
* 4.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Solution A | 500 | ml | ||
|
|
Solution B | 500 | ml | ||
| 1 Sterilize solution A and B separately at 121°C for 15 min. Cool to 50°C and then combine. | |||||
Solution A #
500
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract | 1.00 | g | ||
|
|
(NH4)2SO4 | 0.20 | g | ||
|
|
MgSO4 x 7 H2O | 0.50 | g | ||
|
|
CaCl2 x 2 H2O | 0.25 | g | ||
|
|
KH2PO4 | 0.60 | g | ||
|
|
Distilled water | 500.00 | ml | ||
| 1 Adjust pH to 3.0 - 4.0. | |||||