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DSMZ

DSMZ 1315 . ANAEROBIC THAUERA MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

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Medium for Acidovorax sp. DSM 15015

* Modification: Replace fatty acid by 3,4-dihydroxybenzoate (0.77 g/l)

The modifications for this strain were extracted automatically and have not been curated by experts, yet.
Growth conditions: 28 °C

Main sol. 1315 #

ml
Equipment needed: Autoclave Anaerobic workbench
Compound Amount Unit Conc. [g/L] Conc. [mM]
KNO3 0.85 g 0.85 8.407
NaHCO3 2.52 g 2.52 29.998
CaCl2 0.01 g 0.01 0.09
MgSO4 x 7 H2O 0.50 g 0.5 2.029
MnSO4 0.01 g 0.01 0.066
NH4Cl 0.30 g 0.3 5.609
NaCl 0.05 g 0.05 0.856
Selenite and tungstate solution 2.00 ml - -
Distilled water 884.00 ml - -
Phosphate solution 100.00 ml - -
Acetate solution 98.0 g/L 10.00 ml - -
Vitamin solution 5.00 ml - -
Trace element solution SL-10 1.00 ml - -
3,4-dihydroxybenzoate* 0.77 g/l - -
1 Distribute to gas tight vessels, gas with 90% N2 +10% CO2 to achieve anaerobic conditions and autoclave. After cooling, add the phosphate solution, substrate solution, vitamin solution and trace element solution. After mixing, pH should be 7.1 - 7.5.

Phosphate solution #

100
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 1.45 g 14.5 102.142
KH2PO4 0.25 g 2.5 18.371
Distilled water 100.00 ml - -
1 Distribute to gas tight vessels under N2, autoclave.

Acetate solution 98.0 g/L #

100
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
K-acetate 9.8 g 98 998.55
Distilled water 100.0 ml - -
1 Filter sterilize and gas with N2. For strains DSM 12141, DSM 12142, DSM 11243 and DSM 12144, acetate may be replaced by Na-capronate (stock solution 2.76 g/100 ml).

Vitamin solution #

1000
Solution occurs in 15 other media (first defined in Medium 461).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 50 mg 0.05 0.037
Pantothenic acid 50 mg 0.05 0.228
Riboflavin 50 mg 0.05 0.133
Pyridoxamine hydrochloride 10 mg 0.01 0.049
Biotin 20 mg 0.02 0.082
Folic acid 20 mg 0.02 0.045
Nicotinic acid 25 mg 0.025 0.203
Nicotine amide 25 mg 0.025 0.205
alpha-lipoic acid* 50 mg 0.05 0.242
p-Aminobenzoic acid 50 mg 0.05 0.365
Thiamine-HCl x 2 H2O 50 mg 0.05 0.134
Distilled water 1000 ml - -
1 Stir for some hours, filter sterilize the solution.

Trace element solution SL-10 #

1000
Solution occurs in 266 other media (first defined in Medium 320).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%) 		10.0 ml 2.5 68.568
FeCl2 x 4 H2O 1.5 g 1.5 7.545
ZnCl2 70.0 mg 0.07 0.514
MnCl2 x 4 H2O 100.0 mg 0.1 0.505
H3BO3 6.0 mg 0.006 0.097
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 36.0 mg 0.036 0.149
Distilled water 990.0 ml - -
1 First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.

Selenite and tungstate solution #

1000
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Distilled water 1000.0 ml - -
NaOH 0.5 g 0.5 12.501
Na2SeO3 x 5 H2O 3.0 mg 0.003 0.011
Na2WO4 x 2 H2O 4.0 mg 0.004 0.012
1 First dissolve NaOH, subsequently the metal salts. Distribute to gas tight vessels under N2, autoclave.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.1 - 7.5
Equipment needed: Anaerobic workbench, Autoclave, Filter
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Gas composition

  • 90 % N2
  • 10 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 7
Strains with modifications: 2
Acidovorax sp. DSM 15015

  0.77 g/l 3,4-dihydroxybenzoate
  alpha-lipoic acid

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaHCO3 2.52 29.998
Na2HPO4 x 2 H2O 1.45 10.214
K-acetate 0.98 9.986
KNO3 0.85 8.407
MgSO4 x 7 H2O 0.5 2.029
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Cultivation metadata from 7 strains BacDive

phyla 1 7 strains
oxygen aerobe 6 strains
oxygen obligate aerobe 4 strains
oxygen anaerobe 3 strains
temperature mesophilic 7 strains