1228 .
THIOALKALIVIBRIO THIOCYANODENITRIFICANS MEDIUM
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Selected strain: Thioalkalivibrio thiocyanodenitrificans DSM 16954
This strain uses the standard medium without modifications.
Growth conditions: 7-14 days
aerobic
28 °C
Equipment needed:
Final pH:
10.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2CO3 | 22 | g | ||
|
|
NaHCO3 | 8 | g | ||
|
|
NaCl | 5 | g | ||
|
|
K2HPO4 | 1 | g | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Sterilize at 120°C 20 min in a closed vessel (i.e. a serum tube or bottle) with a liquid to air ratio of about 1:10. pH after sterilization will about 10. | |||||
| 2 After sterilization add from sterile stock solutions: | |||||
|
|
Trace element solution | 2 | ml | ||
|
|
MgCl2 x 6 H2O
(200.0 g/l) |
1 | ml | ||
| 3 (a white colloid will form which will rapidly dissolve after mixing) | |||||
|
|
Sodium thiosulfate pentahydrate
(4 M - 992 g/l) |
10 | ml | ||
|
|
KSCN
(2 M - 194 g/l) |
5 | ml | ||
| 4 Incubate statically until growth occurs, then shake at 100 rpm. | |||||
Trace element solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
EDTA | 5 | mg | ||
|
|
FeSO4 x 7 H2O | 2 | mg | ||
|
|
ZnSO4 x 7 H2O | 100 | mg | ||
|
|
MnCl2 x 4 H2O | 30 | mg | ||
|
|
CoCl2 x 6 H2O | 200 | mg | ||
|
|
NiCl2 x 6 H2O | 20 | mg | ||
|
|
Na2MoO4 x 2 H2O | 30 | mg | ||
|
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CuCl2 x 2 H2O | 10 | mg | ||
|
|
H3BO3 | 300 | mg | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Final pH should be 3, add HCl if needed. Sterilization - 120°C 20 min. | |||||