1203 .
FASTIDIOUS ANAEROBE AGAR
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* Medium for Vermiculatibacterium agrestimuris DSM 112226
solid medium, add. 5% horse blood
Growth conditions: 3-7 days
anaerobic
37 °C
pH 7.00
Equipment needed:
Final pH:
7.0 - 7.4
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Fastidious Anaerobe Agar
(acumedia) |
45.7 | g | ||
|
|
Deionized water | 1000.0 | ml | ||
|
|
Horse blood , adjust if required | 10.0 | % | ||
| 1 Allow to soak for 10 minutes, swirl to mix then sterilize by autoclaving at 121°C for 15 minutes. Cool to 47°C then aseptically add 5-10% of sterile defibrinated horse blood, mix well and pour plates. pH: 7.2 ± 0.2 | |||||