1167 .
NSY-MEDIUM FOR POLYNUCLEOBACTER
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* Medium for Polynucleobacter acidiphobus DSM 21994
Agar surface must be kept moist all the time
Growth conditions: 25 °C
Equipment needed:
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nutrient broth | 1.0 | g | ||
|
|
Soy peptone
(e.g. Bacto Soytone) |
1.0 | g | ||
|
|
Yeast extract | 1.0 | g | ||
|
|
Tap water | 1000.0 | ml | ||
| 1 The following inorganic solution may substitute for tap water: | |||||
|
|
MgSO4 x 7 H2O | 75.0 | mg | ||
|
|
Ca(NO3)2 x 4 H2O | 43.0 | mg | ||
|
|
NaHCO3 | 16.0 | mg | ||
|
|
KCl | 5.0 | mg | ||
|
|
K2HPO4 x 3 H2O | 3.7 | mg | ||
|
|
Distilled water | 1000.0 | ml | ||
|
|
Trace element solution SL-4 | 0.1 | ml | ||
| 2 Adjust to pH 7.2. | |||||
Trace element solution SL-4 #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Na2-EDTA | 0.50 | g | ||
|
|
FeSO4 x 7 H2O | 0.20 | g | ||
|
|
ZnSO4 x 7 H2O | 0.10 | g | ||
|
|
MnCl2 x 4 H2O | 0.03 | g | ||
|
|
H3BO3 | 0.30 | g | ||
|
|
CoCl2 x 6 H2O | 0.20 | g | ||
|
|
CuCl2 x 2 H2O | 0.01 | g | ||
|
|
NiCl2 x 6 H2O | 0.02 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.03 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 First dissolve EDTA in distilled water and adjust pH to 7.0 using 2 N NaOH; then add other compounds. | |||||