104b .
PY + X MEDIUM
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* Medium for Lacrimispora xylanolytica DSM 6555
Replace glucose with 5.0 g/l xylan.
Growth conditions: anaerobic
37 °C
Equipment needed:
Autoclave
Gassing station
Hungate tubes
Final gas composition:
100 % N2
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Final pH:
6.8 - 7.0
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ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Trypticase peptone
(BD BBL) |
5.0 | g | ||
|
|
Meat peptone
(pepsin-digested) |
5.0 | g | ||
|
|
Yeast extract | 10.0 | g | ||
|
|
Salt solution | 40.0 | ml | ||
|
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | ||
|
|
L-Cysteine HCl x H2O | 0.5 | g | ||
| D-Glucose* | 5.0 | g | |||
|
|
Xylan | 5.0 | g | ||
|
|
Distilled water | 960.0 | ml | ||
| 1 Dissolve ingredients (except cysteine and glucose) and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add cysteine and adjust pH to 7.0, then dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After autoclaving add glucose or any other substrate from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Adjust pH of complete medium to 6.8 - 7.0, if necessary. | |||||
Salt solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
CaCl2 x 2 H2O | 0.25 | g |
|
|
MgSO4 x 7 H2O | 0.50 | g |
|
|
K2HPO4 | 1.00 | g |
|
|
KH2PO4 | 1.00 | g |
|
|
NaHCO3 | 10.00 | g |
|
|
NaCl | 2.00 | g |
|
|
Distilled water | 1000.00 | ml |