1002 .
ECTOTHIORHODOSYNUS MEDIUM
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Selected strain: Ectothiorhodosinus mongolicus DSM 15479
This strain uses the standard medium without modifications.
Growth conditions: 30 °C
Equipment needed:
Final pH:
9.0 - 9.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
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KH2PO4 | 0.5 | g | ||
|
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NH4Cl | 0.5 | g | ||
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NaCl | 20.0 | g | ||
|
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MgCl2 x 6 H2O | 0.2 | g | ||
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CaCl2 | 0.1 | g | ||
|
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Na2CO3 | 5.0 | g | ||
|
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NaHCO3 | 5.0 | g | ||
|
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Sodium acetate | 1.0 | g | ||
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Yeast extract | 0.1 | g | ||
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Na2S2O3 x 5 H2O | 0.5 | g | ||
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Na2S x 9 H2O | 0.1 | g | ||
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Trace element solution SL-6 | 1.0 | ml | ||
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Vitamin B12 | 20.0 | µg | ||
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Distilled water | 1000.0 | ml | ||
| 1 Prepare the NH4Cl, CaCl2, carbonate, bicarbonate and sulfide as a single sterile stock solution and add to the sterile medium after autoclaving. The pH should be adjust to 9-9.5. | |||||
Trace element solution SL-6 #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
ZnSO4 x 7 H2O | 0.10 | g |
|
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MnCl2 x 4 H2O | 0.03 | g |
|
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H3BO3 | 0.30 | g |
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CoCl2 x 6 H2O | 0.20 | g |
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CuCl2 x 2 H2O | 0.01 | g |
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NiCl2 x 6 H2O | 0.02 | g |
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Na2MoO4 x 2 H2O | 0.03 | g |
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Distilled water | 1000.00 | ml |