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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.
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Current criteria: Monopotassium Phosphate
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857 BACTO MIDDLEBROOK 7H9 BROTH
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Bovine albumin | 5 | - |
| Disodium Phosphate | 2.5 | 17.611 |
| Dextrose | 2 | 10.092 |
| Monopotassium Phosphate | 1 | 7.348 |
| Ammonium Sulfate | 0.5 | 3.784 |
| L-Glutamic acid | 0.5 | 3.398 |
| Sodium citrate | 0.1 | 0.387 |
| Magnesium sulfate | 0.05 | 0.415 |
| Ferric ammonium citrate | 0.04 | - |
| Copper Sulfate | 1.0e-3 | 0.006 |
| Zinc Sulfate | 1.0e-3 | 0.006 |
| Pyridoxine | 1.0e-3 | 0.006 |
| Calcium Chloride | 5.0e-4 | 0.003 |
| Biotin | 5.0e-4 | 0.002 |
| Catalase | 3.0e-4 | - |
| Distilled water | - | - |
Solutions:
- 100 ml ADC Enrichment
1634 SERPENTINOMONAS MINIMAL MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| CAPS buffer | 3.32 | 15.001 |
| CaCl2 x 2 H2O | 0.735 | 5 |
| Na acetate | 0.328 | 4 |
| NH4Cl | 0.08 | 1.499 |
| K2HPO4 | 0.052 | 0.3 |
| MgCl2 x 6 H2O | 0.04 | 0.199 |
| MgSO4 x 7 H2O | 0.03 | 0.122 |
| Na2SO4 | 0.028 | 0.2 |
| NaCl | 0.01 | 0.171 |
| Monopotassium Phosphate | 0.009 | 0.066 |
| MnSO4 x H2O | 0.005 | 0.03 |
| Na-EDTA x 2 H2O | 0.005 | 0.015 |
| CaCl2 | 1.0e-3 | 0.009 |
| FeSO4 x 7 H2O | 1.0e-3 | 0.004 |
| ZnSO4 x 7 H2O | 1.0e-3 | 0.003 |
| Co(NO3)2 x 6 H2O | 1.0e-3 | 0.003 |
| NiCl2 x 6 H2O | 2.0e-4 | 0.002 |
| H3BO3 | 1.0e-4 | 0.002 |
| Pyridoxine hydrochloride | 1.0e-4 | 4.9e-4 |
| Na2 MoO4 x 2 H2O | 1.0e-4 | 4.1e-4 |
| CuSO4 x 5 H2O | 1.0e-4 | 4.0e-4 |
| AlK(SO4)2 | 1.0e-4 | 3.9e-4 |
| Na2WO4 x 2 H2O | 1.0e-4 | 3.0e-4 |
| Nicotinic acid | 5.0e-5 | 4.1e-4 |
| p-Aminobenzoic acid | 5.0e-5 | 3.6e-4 |
| Thioctic acid | 5.0e-5 | 2.4e-4 |
| Thiamine | 5.0e-5 | 1.9e-4 |
| Riboflavin | 5.0e-5 | 1.3e-4 |
| Calcium pantothenate | 5.0e-5 | 1.0e-4 |
| Biotin | 2.0e-5 | 8.2e-5 |
| Folic acid | 2.0e-5 | 4.5e-5 |
| Na2SeO3 | 1.0e-5 | 5.8e-5 |
| Vitamin B12 | 1.0e-6 | 7.4e-7 |
| Double distilled water | - | - |
| Distilled water | - | - |
Solutions:
- 10 ml Vitamin solution
- 10 ml Trace element solution
Gas phase:
- 70 % N2
- 30 % Air