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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.



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465d MINERAL MEDIUM WITH BENZYLCYANIDE AS NITROGEN SOURCE

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 3.5 24.655
Glucose 1.8 9.991
KH2PO4 1 7.348
Benzylcyanide 0.12 -
MgCl2 x 6 H2O 0.1 0.492
Ca(NO3)2 x 4 H2O 0.05 0.212
EDTA 5.0e-4 0.002
FeSO4 x 7 H2O 2.0e-4 7.2e-4
H3BO3 3.0e-5 4.9e-4
CoCl2 x 6 H2O 2.0e-5 1.5e-4
ZnSO4 x 7 H2O 1.0e-5 3.5e-5
MnCl2 x 4 H2O 3.0e-6 1.5e-5
Na2MoO4 x 2 H2O 3.0e-6 1.2e-5
NiCl2 x 6 H2O 2.0e-6 1.5e-5
CuCl2 x 2 H2O 1.0e-6 5.9e-6
Distilled water - -
Solutions:
  • 1000 ml Main sol. 465 (modified)
  • 1 ml Trace element solution SL-4