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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.
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Current criteria: 6-methylnicotinate
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465h MINERALMEDIUM WITH 6-METHYLNICOTINATE
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| 6-methylnicotinate | 5 | - |
| Na2HPO4 x 2 H2O | 3.5 | 24.655 |
| KH2PO4 | 1 | 7.348 |
| (NH4)2SO4 | 0.5 | 3.784 |
| MgCl2 x 6 H2O | 0.1 | 0.492 |
| Ca(NO3)2 x 4 H2O | 0.05 | 0.212 |
| NaOH | 5.0e-4 | 0.013 |
| EDTA | 5.0e-4 | 0.002 |
| FeSO4 x 7 H2O | 2.0e-4 | 7.2e-4 |
| p-Aminobenzoic acid | 5.0e-5 | 3.6e-4 |
| alpha-lipoic acid | 5.0e-5 | 2.4e-4 |
| Pantothenic acid | 5.0e-5 | 2.3e-4 |
| Thiamine-HCl x 2 H2O | 5.0e-5 | 1.3e-4 |
| Riboflavin | 5.0e-5 | 1.3e-4 |
| Vitamin B12 | 5.0e-5 | 3.7e-5 |
| H3BO3 | 3.0e-5 | 4.9e-4 |
| Nicotine amide | 2.5e-5 | 2.0e-4 |
| Nicotinic acid | 2.5e-5 | 2.0e-4 |
| CoCl2 x 6 H2O | 2.0e-5 | 1.5e-4 |
| Biotin | 2.0e-5 | 8.2e-5 |
| Folic acid | 2.0e-5 | 4.5e-5 |
| Pyridoxamine hydrochloride | 1.0e-5 | 4.9e-5 |
| ZnSO4 x 7 H2O | 1.0e-5 | 3.5e-5 |
| Na2WO4 x 2 H2O | 4.0e-6 | 1.2e-5 |
| MnCl2 x 4 H2O | 3.0e-6 | 1.5e-5 |
| Na2MoO4 x 2 H2O | 3.0e-6 | 1.2e-5 |
| Na2SeO3 x 5 H2O | 3.0e-6 | 1.1e-5 |
| NiCl2 x 6 H2O | 2.0e-6 | 1.5e-5 |
| CuCl2 x 2 H2O | 1.0e-6 | 5.9e-6 |
| Distilled water | - | - |
Solutions:
- 1 ml Trace element solution SL-4
- 1 ml Selenite/tungstate stock solution
- 1 ml Vitamin solution