# J699: MODIFIED THIOCAPSA MEDIUM


## Main sol. J699

1	g	NaCl	
0.4	g	MgCl2 x 6 H2O	
0.2	g	KH2PO4	
0.5	g	KCl	
0.125	g	NH4Cl	
0.15	g	CaCl2 x 2 H2O	
0.15	g	Na2SO4	
0.069	g	NaNO2	
1	ml	**Modified trace element solution SL-12**	
1	ml	**Seven vitamins solution**	
950	ml	Distilled water	

1. Add NaHCO3 buffer (see below) and adjust pH to 7.2 - 7.4, if necessary.


## NaHCO3 buffer

2.5	g	NaHCO3	
50	ml	Distilled water	


## Modified trace element solution SL-12

300	mg	H3BO3	
1	g	FeSO4 x 7 H2O	
190	mg	CoCl2 x 6 H2O	
100	mg	MnCl2 x 4 H2O	
70	mg	ZnCl2	
24	mg	NiCl2 x 6 H2O	
18	mg	Na2MoO4 x 2 H2O	
2	mg	CuCl2 x 2 H2O	
1000	ml	Distilled water	


## Seven vitamins solution

10	mg	Vitamin B12	
10	mg	p-Aminobenzoic acid	
2	mg	D-(+)-biotin	
20	mg	Nicotinic acid	
5	mg	Calcium pantothenate	
50	mg	Pyridoxine hydrochloride	
10	mg	Thiamine-HCl x 2 H2O	
200	ml	Distilled water