# J434: ANAEROLINEA MEDIUM


## Main sol. J434

1. Use Medium No. 284 supplemented with final 1.0 ml Trace vitamins solution in Solution A (per 900 ml), and with 7.2 g sucrose in Solution B (per 100 ml) instead of sodium pyruvate. Otherwise, prepare the medium as described in the recipe of Medium No. 284.
2. Comment: For strains JCM 12577 and JCM 12578, incubate with a 10% inoculum.