# J237: THERMOTOGA MEDIUM


## Main sol. J237

5	g	Starch (soluble)	
0.5	g	KH2PO4	
15	ml	**Trace minerals**	
2	mg	NiCl2 x 6 H2O	
20	g	NaCl	
0.5	g	Yeast extract (BD-Difco)	
1	mg	Resazurin	
0.5	g	Na2S x 9 H2O	
250	ml	**Artificial sea water**	
750	ml	Distilled water	

1. Mix components except Na2S x 9H2O, adjust pH to 6.5 and autoclave under a N2 atmosphere. Separately autoclave a 5% Na2S x 9H2O solution (pH 7.0) under a N2 atmosphere. Prior to inoculation, add the sterile Na2S x 9H2O solution to the medium.


## Artificial sea water

27.7	g	NaCl	
7	g	MgSO4 x 7 H2O	
5.5	g	MgCl2 x 6 H2O	
0.65	g	KCl	
0.1	g	NaBr	
30	mg	H3BO3	
15	mg	SrCl2 x 6 H2O	
10	mg	Citric acid	
0.05	mg	KI	
2.25	g	CaCl2 x 2 H2O	
1000	ml	Distilled water	


## Trace minerals

1.5	g	Nitrilotriacetic acid	
3	g	MgSO4 x 7 H2O	
0.5	g	MnSO4 x n H2O	
1	g	NaCl	
0.1	g	FeSO4 x 7 H2O	
0.1	g	CoSO4 x 7 H2O	
0.1	g	CaCl2 x 2 H2O	
0.1	g	ZnSO4 x 7 H2O	
0.01	g	CuSO4 x 5 H2O	
0.01	g	AlK(SO4)2	
0.01	g	H3BO3	
0.01	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	

1. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.