# J171: SULFOLOBUS SOLFATARICUS MEDIUM


## Main sol. J171

3.1	g	KH2PO4	
2.5	g	(NH4)2SO4	
0.2	g	MgSO4 x 7 H2O	
0.25	g	CaCl2 x 2 H2O	
1.8	mg	MnCl2 x 4 H2O	
4.5	mg	Na2B4O7 x 10 H2O	
0.22	mg	ZnSO4 x 7 H2O	
0.05	mg	CuCl2 x 2 H2O	
0.03	mg	Na2MoO4 x 2 H2O	
0.03	mg	VOSO4 x n H2O	
0.01	mg	CoSO4 x 7 H2O	
1	g	Yeast extract (BD-Difco)	
1	g	Casamino acids (BD-Difco)	
1000	ml	Distilled water	

1. Adjust pH to 4.0-4.2 with 10 N H2SO4.