# J1318: YCFA MEDIUM WITH 10% RUMEN FLUID


## Main sol. J1318

1. Use Medium No. 1130 with 10% rumen fluid, clarified (see below).
2. To prepare the clarified rumen fluid, preheat rumen content at 121C for 15 min. Use the supernatant after centrifuging at 25,000 x g for 15 min.