# J13: BL AGAR (GLUCOSE BLOOD LIVER AGAR)


## Main sol. J13

3	g	Lab-Lemco beef extract (Oxoid)	
10	g	Proteose peptone no. 3 (BD-Difco)	
5	g	Trypticase peptone (BD-BBL)	
3	g	Phytone peptone (BD-BBL)	
5	g	Yeast extract (BD-Difco)	
150	ml	Liver extract (see below)	
10	g	Glucose	
0.5	g	Starch (soluble)	
10	ml	**Solution A**	
5	ml	**Solution B**	
1	g	Tween 80	
15	g	Agar (BD-Difco, Bacto )	
10	ml	L-Cysteine HCl x H2O (5%)	
50	ml	Horse blood	
825	ml	Distilled water	

1. Adjust pH to 7.2.
2. To prepare liver extract, put 10 g liver powder in 170 ml water, keep at 50 to 60C for 1 hr, boil for 5 min, adjust pH to 7.2 and filter.


## Solution A

10	g	K2HPO4	
10	g	KH2PO4	
100	ml	Distilled water	


## Solution B

4	g	MgSO4 x 7 H2O	
0.2	g	NaCl	
0.2	g	FeSO4 x 7 H2O	
0.2	g	MnSO4 x n H2O	
100	ml	Distilled water	

1. Mix ingredients except horse blood and adjust pH to 7.2. After autoclaving, cool to 50C, and aseptically add 50.0 ml of horse blood.
2. Comment: Commercial BL agar (Nissui) is also available.