# J1191: ALKALINE TRYPTICASE SOY BROTH AGAR ## Main sol. J1191 30 g Trypticase soy broth (BD-BBL) 15 g Agar 900 ml Distilled water 1. After autoclaving, aseptically add 100 ml of a sterile 10% Na2CO3 solution to the medium. Check final pH to be about 10.0.