# 852: GYP GLUCOSE-YEAST-PEPTONE MEDIUM


## Main sol. 852

20	g	Glucose	
10	g	Yeast extract	
10	g	Peptone (tryptic)	
10	g	Na-acetate	
5	ml	**Salt solution**	
1000	ml	Distilled water	
10	g	Agar, for solid medium	

1. For solid media add 10 g/l bacteriological agar. Adjust to pH 6.8. After sterilization of the medium add filter sterilized salt solution.


## Salt solution

40	g	MgSO4 x 7 H2O	
2	g	MnSO4 x 4 H2O	
2	g	FeSO4 x 7 H2O	
2	g	NaCl	
1000	ml	Distilled water