# 830c: REACTIVATION WITH LIQUID MEDIUM 830


## Main sol. 830c

1000	ml	**830. R2A MEDIUM**	

1. Rehydrate and grow lyophilized cells from ampoules in liquid broth 830. Subsequent subculturing may be carried out in liquid medium or on agar plates.


## 830. R2A MEDIUM

0.5	g	Yeast extract	
0.5	g	Proteose peptone (Difco no. 3)	
0.5	g	Casamino acids	
0.5	g	Glucose	
0.5	g	Starch (soluble)	
0.3	g	Na-pyruvate	
0.3	g	K2HPO4	
0.05	g	MgSO4 x 7 H2O	
15	g	Agar	
1000	ml	Distilled water	

1. Final pH 7.2; adjust with crystalline K2HPO4 or KH2PO4 before adding agar. Add agar, heat medium to boiling to dissolve agar, and autoclave for 15 min at 121°C.