# 756d: NITROSPIRA MOSCOVIENSIS


## Main sol. 756d

1	ml	**Trace element solution**	
100	ml	**Stock solution**	
0.5	g	NaNO2	
899	ml	Distilled water	

1. Adjust pH to 8.6 with NaOH or KOH. Autoclave and leave the medium to stand for 2 - 3 days so that pH can ajust itself to pH 7.4 - 7.6 .


## Trace element solution

33.8	mg	MnSO4 x H2O	
49.4	mg	H3BO3	
43.1	mg	ZnSO4 x 7 H2O	
37.1	mg	(NH4)6Mo7O24	
97.3	mg	FeSO4 x 7 H2O	
25	mg	CuSO4 x 5 H2O	
1000	ml	Distilled water	


## Stock solution

0.07	g	CaCO3	
5	g	NaCl	
0.5	g	MgSO4 x 7 H2O	
1.5	g	KH2PO4	
1000	ml	Distilled water