# 60: MOORELLA MEDIUM


## Main sol. 60

843	ml	**Solution A**	
50	ml	**Solution B**	
50	ml	**Solution C**	
40	ml	**Solution D**	
10	ml	**Solution E**	
10	ml	**Solution F**	

1. Sparge solution A with 100% CO2 gas for 30 - 45 min to make it anoxic, then distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Autoclave separately solutions B, C, E and F under 100% N2 gas, and solution D under 80% N2 and 20% CO2 gas atmosphere. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Adjust pH of the complete medium to 6.9, if necessary.


## Solution A

0.1	g	MgSO4 x 7 H2O	
0.5	g	(NH4)2SO4	
0.04	g	Fe(NH4)2(SO4)2	
2.4	ml	Na2MoO4 x 2 H2O (0.1% w/v)	
0.15	ml	Na2SeO3 x 5 H2O (0.1% w/v)	
5	g	Tryptone	
5	g	Yeast extract	
0.5	ml	Sodium resazurin (0.1% w/v)	
840	ml	Distilled water	


## Solution B

7	g	K2HPO4	
4.5	g	KH2PO4	
50	ml	Distilled water	


## Solution C

18	g	Glucose	
50	ml	Distilled water	


## Solution D

2	g	Na2CO3	
40	ml	Distilled water	


## Solution E

0.3	g	L-Cysteine HCl x H2O	
10	ml	Distilled water	


## Solution F

0.3	g	Na2S x 9 H2O	
10	ml	Distilled water