# 531: SPORULATION MEDIUM ## Main sol. 531 6 g Nutrient broth (Difco) 1 g Yeast extract 2 g Trypticase (BBL) 0.5 mg MnSO4 x H2O 0.5 g Marine Broth (Difco) 10 g Agar 1 ml Inosin (5 mM) 1000 ml Distilled water