# 514c: REACTIVATION WITH LIQUID MEDIUM 514


## Main sol. 514c

1000	ml	**514. BACTO MARINE BROTH (DIFCO 2216)**	

1. Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid medium 514. Subsequent subculturing may be carried out in liquid medium or on agar plates.


## 514. BACTO MARINE BROTH (DIFCO 2216)

5	g	Bacto peptone	
1	g	Yeast extract (Bacto )	
0.1	g	Fe(III) citrate	
19.45	g	NaCl	
5.9	g	MgCl2 (dried)	
3.24	g	Na2SO4	
1.8	g	CaCl2	
0.55	g	KCl	
0.16	g	Na2CO3	
0.08	g	KBr	
34	mg	SrCl2	
22	mg	H3BO3	
4	mg	Na-silicate	
2.4	mg	NaF	
1.6	mg	(NH4)NO3	
8	mg	Na2HPO4	
18	g	Agar	
1000	ml	Distilled water	

1. Final pH should be 7.6 ± 0.2 at 25°C. If using the complete medium from Difco add 37.4 g to 1 litre water.