# 464a: REACTIVATION WITH LIQUID MEDIUM 464


## Main sol. 464a

1000	ml	**Main sol. 464**	

1. Rehydrate and grow lyophilized cells from the ampoule in liquid medium 464. Subsequently subculturing may be carried out in liquid medium or an agar medium.


## Main sol. 464

5	g	Tryptone	
2.5	g	Yeast extract	
1	g	Dextrose	
15	g	Agar	
1000	ml	Distilled water	

1. Adjust pH to 7.0.