# 463a: EDTA-MEDIUM


## Main sol. 463a

0.41	g	Na2HPO4 x 2 H2O	
0.26	g	KH2PO4	
1	g	MgSO4 x 7 H2O	
0.4	g	CaCl2 x 2 H2O	
1	ml	**Trace element solution**	
1	ml	**Vitamin solution**	
0.5	g	EDTA (di- or trisodium salt)	
1000	ml	Distilled water	

1. pH 8.0


## Trace element solution

1.5	g	FeCl2 x 4 H2O	
68	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
62	mg	H3BO3	
120	mg	CoCl2 x 6 H2O	
17	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
24	mg	Na2MoO4 x 2 H2O	
1000	ml	HCl, 0.05 molar	


## Vitamin solution

50	mg	Vitamin B12	
50	mg	Pantothenic acid	
50	mg	Riboflavin	
10	mg	Pyridoxamine hydrochloride	
20	mg	Biotin	
20	mg	Folic acid	
25	mg	Nicotinic acid	
25	mg	Nicotine amide	
50	mg	alpha-lipoic acid	
50	mg	p-Aminobenzoic acid	
50	mg	Thiamine-HCl x 2 H2O	
1000	ml	Distilled water	

1. Stir for some hours, filter sterilize the solution.