# 419: OXALOBACTER MEDIUM


## Main sol. 419

0.25	g	K2HPO4	
0.25	g	KH2PO4	
0.5	g	(NH4)2SO4	
25	mg	MgSO4 x 7 H2O	
1	ml	**Trace element solution SL-10**	
1	ml	**Selenite-tungstate solution**	
5	g	Na-oxalate	
0.82	g	Na-acetate	
1	g	Yeast extract (OXOID)	
1	g	Trypticase peptone (BD BBL)	
0.5	ml	Sodium resazurin (0.1% w/v)	
4	g	Na2CO3	
0.5	g	L-Cysteine HCl x H2O	
1000	ml	Distilled water	

1. Dissolve ingredients except carbonate and cysteine, adjust pH to 6.8, bring medium to the boil and then cool to room temperature under 100% CO2 gas atmosphere. Add solid carbonate and cysteine, then equilibrate pH to 6.8. Dispense under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave.


## Trace element solution SL-10

10	ml	HCl (25%)	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
990	ml	Distilled water	

1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.


## Selenite-tungstate solution

0.5	g	NaOH	
3	mg	Na2SeO3 x 5 H2O	
4	mg	Na2WO4 x 2 H2O	
1000	ml	Distilled water